Review



eb3 gfp tracks  (Oxford Instruments)


Bioz Verified Symbol Oxford Instruments is a verified supplier
Bioz Manufacturer Symbol Oxford Instruments manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Oxford Instruments eb3 gfp tracks
    <t>EB3-GFP</t> tracks show plus-ends of microtubules move to the cell base A) Example image of a neuromast at 2 dpf. The growing or plus-ends of microtubules are marked with EB3-GFP. In this example the apex of 8 developing cells is at the center of the image and the base of each cell is at the periphery. 2 example cells are outlined (dashed lines) and expanded in more detail in in C-D, and E-F . B) A 22 min timelapse was taken of the example in A . All EB3-tracks, indicated by magenta arrows (Tracked in Imaris) acquired during the timelapse are shown. C-D) Example time courses of EB3-GFP in hair cells over 21 s; the cell apex is towards the top of each image. In the final image, the 4 images for each example (0-21 s) were projected overtime as a pseudocolor image represented by the colormap. The pseudocolor images show that many EB3-GFP tracks move to the cell base. E-F) The magenta arrows in E and F show all the EB3-GFP tracks acquired in the example cells in C-D over the entire 22 min duration. Arrowheads indicate direction of travel. G) The schematic in G shows how EB3-GFP tracks were aligned to each hair cell. Tracks moving toward the apex have a track angle of 180°, while those moving to the base have an angle of 0°. This analysis revealed that the majority of EB3-GFP tracks (shaded domains) move toward the base of the cell (n = 7 neuromasts, 2597 tracks). Scale bars in A = 5 µm and in C-F = 2 µm.
    Eb3 Gfp Tracks, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eb3+gfp+tracks/Imaris/bio_rxiv__2024__04__12__589161-103-5-9
    Average 99 stars, based on 44453 article reviews
    eb3 gfp tracks - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Microtubule networks in zebrafish hair cells facilitate presynapse transport and fusion during development"

    Article Title: Microtubule networks in zebrafish hair cells facilitate presynapse transport and fusion during development

    Journal: bioRxiv

    doi: 10.1101/2024.04.12.589161

    EB3-GFP tracks show plus-ends of microtubules move to the cell base A) Example image of a neuromast at 2 dpf. The growing or plus-ends of microtubules are marked with EB3-GFP. In this example the apex of 8 developing cells is at the center of the image and the base of each cell is at the periphery. 2 example cells are outlined (dashed lines) and expanded in more detail in in C-D, and E-F . B) A 22 min timelapse was taken of the example in A . All EB3-tracks, indicated by magenta arrows (Tracked in Imaris) acquired during the timelapse are shown. C-D) Example time courses of EB3-GFP in hair cells over 21 s; the cell apex is towards the top of each image. In the final image, the 4 images for each example (0-21 s) were projected overtime as a pseudocolor image represented by the colormap. The pseudocolor images show that many EB3-GFP tracks move to the cell base. E-F) The magenta arrows in E and F show all the EB3-GFP tracks acquired in the example cells in C-D over the entire 22 min duration. Arrowheads indicate direction of travel. G) The schematic in G shows how EB3-GFP tracks were aligned to each hair cell. Tracks moving toward the apex have a track angle of 180°, while those moving to the base have an angle of 0°. This analysis revealed that the majority of EB3-GFP tracks (shaded domains) move toward the base of the cell (n = 7 neuromasts, 2597 tracks). Scale bars in A = 5 µm and in C-F = 2 µm.
    Figure Legend Snippet: EB3-GFP tracks show plus-ends of microtubules move to the cell base A) Example image of a neuromast at 2 dpf. The growing or plus-ends of microtubules are marked with EB3-GFP. In this example the apex of 8 developing cells is at the center of the image and the base of each cell is at the periphery. 2 example cells are outlined (dashed lines) and expanded in more detail in in C-D, and E-F . B) A 22 min timelapse was taken of the example in A . All EB3-tracks, indicated by magenta arrows (Tracked in Imaris) acquired during the timelapse are shown. C-D) Example time courses of EB3-GFP in hair cells over 21 s; the cell apex is towards the top of each image. In the final image, the 4 images for each example (0-21 s) were projected overtime as a pseudocolor image represented by the colormap. The pseudocolor images show that many EB3-GFP tracks move to the cell base. E-F) The magenta arrows in E and F show all the EB3-GFP tracks acquired in the example cells in C-D over the entire 22 min duration. Arrowheads indicate direction of travel. G) The schematic in G shows how EB3-GFP tracks were aligned to each hair cell. Tracks moving toward the apex have a track angle of 180°, while those moving to the base have an angle of 0°. This analysis revealed that the majority of EB3-GFP tracks (shaded domains) move toward the base of the cell (n = 7 neuromasts, 2597 tracks). Scale bars in A = 5 µm and in C-F = 2 µm.

    Techniques Used:

    Related Articles

    other:

    Article Title: Microtubule networks in zebrafish hair cells facilitate presynapse transport and fusion during development
    Article Snippet: To quantify the direction of EB3-GFP tracks we used Imaris to perform 3D analyses to detect and create vectors of EB3-GFP tracks (see arrows; ,F).



    Similar Products

    eb3 gfp tracks  (Oxford Instruments)
    99
    Oxford Instruments eb3 gfp tracks
    <t>EB3-GFP</t> tracks show plus-ends of microtubules move to the cell base A) Example image of a neuromast at 2 dpf. The growing or plus-ends of microtubules are marked with EB3-GFP. In this example the apex of 8 developing cells is at the center of the image and the base of each cell is at the periphery. 2 example cells are outlined (dashed lines) and expanded in more detail in in C-D, and E-F . B) A 22 min timelapse was taken of the example in A . All EB3-tracks, indicated by magenta arrows (Tracked in Imaris) acquired during the timelapse are shown. C-D) Example time courses of EB3-GFP in hair cells over 21 s; the cell apex is towards the top of each image. In the final image, the 4 images for each example (0-21 s) were projected overtime as a pseudocolor image represented by the colormap. The pseudocolor images show that many EB3-GFP tracks move to the cell base. E-F) The magenta arrows in E and F show all the EB3-GFP tracks acquired in the example cells in C-D over the entire 22 min duration. Arrowheads indicate direction of travel. G) The schematic in G shows how EB3-GFP tracks were aligned to each hair cell. Tracks moving toward the apex have a track angle of 180°, while those moving to the base have an angle of 0°. This analysis revealed that the majority of EB3-GFP tracks (shaded domains) move toward the base of the cell (n = 7 neuromasts, 2597 tracks). Scale bars in A = 5 µm and in C-F = 2 µm.
    Eb3 Gfp Tracks, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eb3+gfp+tracks/Imaris/bio_rxiv__2024__04__12__589161-103-5-9
    Average 99 stars, based on 1 article reviews
    eb3 gfp tracks - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    EB3-GFP tracks show plus-ends of microtubules move to the cell base A) Example image of a neuromast at 2 dpf. The growing or plus-ends of microtubules are marked with EB3-GFP. In this example the apex of 8 developing cells is at the center of the image and the base of each cell is at the periphery. 2 example cells are outlined (dashed lines) and expanded in more detail in in C-D, and E-F . B) A 22 min timelapse was taken of the example in A . All EB3-tracks, indicated by magenta arrows (Tracked in Imaris) acquired during the timelapse are shown. C-D) Example time courses of EB3-GFP in hair cells over 21 s; the cell apex is towards the top of each image. In the final image, the 4 images for each example (0-21 s) were projected overtime as a pseudocolor image represented by the colormap. The pseudocolor images show that many EB3-GFP tracks move to the cell base. E-F) The magenta arrows in E and F show all the EB3-GFP tracks acquired in the example cells in C-D over the entire 22 min duration. Arrowheads indicate direction of travel. G) The schematic in G shows how EB3-GFP tracks were aligned to each hair cell. Tracks moving toward the apex have a track angle of 180°, while those moving to the base have an angle of 0°. This analysis revealed that the majority of EB3-GFP tracks (shaded domains) move toward the base of the cell (n = 7 neuromasts, 2597 tracks). Scale bars in A = 5 µm and in C-F = 2 µm.

    Journal: bioRxiv

    Article Title: Microtubule networks in zebrafish hair cells facilitate presynapse transport and fusion during development

    doi: 10.1101/2024.04.12.589161

    Figure Lengend Snippet: EB3-GFP tracks show plus-ends of microtubules move to the cell base A) Example image of a neuromast at 2 dpf. The growing or plus-ends of microtubules are marked with EB3-GFP. In this example the apex of 8 developing cells is at the center of the image and the base of each cell is at the periphery. 2 example cells are outlined (dashed lines) and expanded in more detail in in C-D, and E-F . B) A 22 min timelapse was taken of the example in A . All EB3-tracks, indicated by magenta arrows (Tracked in Imaris) acquired during the timelapse are shown. C-D) Example time courses of EB3-GFP in hair cells over 21 s; the cell apex is towards the top of each image. In the final image, the 4 images for each example (0-21 s) were projected overtime as a pseudocolor image represented by the colormap. The pseudocolor images show that many EB3-GFP tracks move to the cell base. E-F) The magenta arrows in E and F show all the EB3-GFP tracks acquired in the example cells in C-D over the entire 22 min duration. Arrowheads indicate direction of travel. G) The schematic in G shows how EB3-GFP tracks were aligned to each hair cell. Tracks moving toward the apex have a track angle of 180°, while those moving to the base have an angle of 0°. This analysis revealed that the majority of EB3-GFP tracks (shaded domains) move toward the base of the cell (n = 7 neuromasts, 2597 tracks). Scale bars in A = 5 µm and in C-F = 2 µm.

    Article Snippet: To quantify the direction of EB3-GFP tracks we used Imaris to perform 3D analyses to detect and create vectors of EB3-GFP tracks (see arrows; ,F).

    Techniques: